Hidayatullah, Permata Ika Hidayati (2011) ARTIKEL SEMINAR INTERNASIONAL ICOMSC2011. PROCEEDING INTERNATIONAL CONFERENCE MATHEMATICS AND SCIENCE FOR IMPROVING HUMAN WELFARE. ISSN 978-602-19142-0-5

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Abstract

Abstract. Rastrelliger canagurta are one kind of fish that easily get damage because of their spacious body tissue, so that the microbe can use the fish flesh for their substrat. Therefore it is need a special handling in order to prevent damaged and putrided. One of the biologycal preservation method for fish is ensiling fermentation preservation, it is a preservation that use vegetable, i.e. : cabbage lettuce (Lactucca sativa) leaf dan kepayang seed (Pangium edule Reinw). Proteolitic and lipolytic bacterias that found in fish body have a potention to putrid the fish flesh. The purpose of this research are: 1) to isolate the bacterias in preserved Rastrelliger canagurta; 2) to know the proteolytic bacterias in preserved Rastrelliger canagurta; 3) to know the lipolytic bacterias in preserved Rastrelliger canagurta. Rastrelliger canagurta samples take from markets in Malang city. Fish samples preserved by combine cabbage lettuce (Lactucca sativa) ensiling fermentation dan kepayang seed (Pangium edule Reinw) then saved for 21 days. 25 grams Rastrelliger canagurta sample diluted in 225 ml peptone broth 0.1% solution to get 10-1 dilution factor of suspension. Then the suspension diluted gradually to get 10-2, 10-3, 10-4, 10-5, and 10-6 dilution factor of suspension. Each suspension inoculated 0.1 ml on Plate Count Agar (PCA) medium and incubated in 370C during 1-2x24 hours. Every bacteria species that grow on PCA medium were isolated, then proteolytic and lipolytic characters of each bacteria were confirmed. Protein hydrolysis index and lipid hydrolysis index were counted. The research result shows: 1) there are 9 isolate of bacterias found in the fish samples; 2) there are two isolate of proteolytic bacteria in preserved Rastrelliger canagurta; 3) there are nine isolate of lipolytic bacteria in preserved Rastrelliger canagurta. Keywords: Rastrelliger canagurta, cabbage lettuce leaf, kepayang seed, proteolytic bacteria, lipolytic bacteria.

Item Type:	Article
Divisions:	Fakultas Peternakan > Peternakan
Depositing User:	Dr., SPi., PERMATA IKA HIDAYATI hidayatullah
Date Deposited:	29 Sep 2016 03:31
Last Modified:	08 Nov 2022 03:23
URI:	http://repository.unikama.ac.id/id/eprint/652

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